The HELP-UnaG Fusion Protein as a Bilirubin Biosensor: From Theory to Mature Technological Development
HUG is the HELP-UnaG recombinant fusion protein featuring the typical functions of both HELP and TMG UnaG.In HUG, the HELP domain is a thermoresponsive human elastin-like polypeptide.It forms a shield enwrapping the UnaG domain that emits bilirubin-dependent fluorescence.Here, we recapitulate the technological development of this bifunctional synth